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Introduction of the PXXP motif in the ABD of p110α induces a gain of function. (A) Oncogenic activity as measured by transformation of <t>CEF.</t> Representative photographs of cell cultures transfected with 0.5 µg/well of the RCAS(A) vector expressing the respective p110 pro- teins. (B) Integrated band intensities of p-S6 staining of <t>CEFs</t> that express the indicated RCAS constructs. * p < 0.05, ** p <0.01, *** p < 0.005
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Introduction of the PXXP motif in the ABD of p110α induces a gain of function. (A) Oncogenic activity as measured by transformation of CEF. Representative photographs of cell cultures transfected with 0.5 µg/well of the RCAS(A) vector expressing the respective p110 pro- teins. (B) Integrated band intensities of p-S6 staining of CEFs that express the indicated RCAS constructs. * p < 0.05, ** p <0.01, *** p < 0.005

Journal: bioRxiv

Article Title: The constitutive oncogenic and signaling activities of phosphatidylinositol 3-kinase (PI3K) isoforms p110β and p110δ

doi: 10.1101/2025.06.26.659600

Figure Lengend Snippet: Introduction of the PXXP motif in the ABD of p110α induces a gain of function. (A) Oncogenic activity as measured by transformation of CEF. Representative photographs of cell cultures transfected with 0.5 µg/well of the RCAS(A) vector expressing the respective p110 pro- teins. (B) Integrated band intensities of p-S6 staining of CEFs that express the indicated RCAS constructs. * p < 0.05, ** p <0.01, *** p < 0.005

Article Snippet: Chicken embryo fibroblasts (CEF) were prepared from single, pathogen-free White Leghorn em- bryos (Charles River, N. Franklin, CT) as previously described [ ].

Techniques: Activity Assay, Transformation Assay, Transfection, Plasmid Preparation, Expressing, Staining, Construct